MitoBOX: "The mitochonrial basis of hypoxia tolerance in marine mollusks"
Supervisor: Prof. Dr. Inna Sokolova (UHRO), Dr. Christian Bock (AWI)
Projektförderung: Deutsche Forschungsgemeinschaft (DFG)
Laufzeit: 01.04.2019 - 31.03.2022
Therefore, short-term hypoxia and subsequent reoxygenation incubations on five marine bivalve species (Ostrea edulis, Crassostrea gigas, Mytilus edulis, Arctica islandica, Pecten maximus) covering a broad range of hypoxia tolerance are used to identify possible adaptive traits. We use the top-down metabolic control analysis to assess effects of hypoxia/reoxygenation stress on the capacity of mitochondrial subsystems and their control over mitochondrial respiration, ATP synthesis and ROS production. Additional mRNA expression, immunoassays and activity of key mitochondrial enzymes are analysed to determine molecular mitochondrial quality control during H/R stress. For a better understanding of the interplay of whole organisms’ resilience and mitochondrial functions, additional in vivo MRI (magnetic resonance imaging) and in vitro NMR spectroscopy are conducted. The combination of mitochondrial and molecular insights as well as in vitro and in vivo studies are expected to uncover important mechanisms of mitochondrial and whole organisms’ resilience during oxygen fluctuations